SYNERGISTIC ANTIPROLIFERATIVE ACTIVITY OF PENNISETUM GLAUCUM (PEARL MILLET) AND TAMARINDUS INDICA EXTRACTS ON CANCEROUS CELL LINES

NGULE CHRISPUS MUTUKU

Abstract


Abstract

Background: Cancer is a dreadful human disease that is on the rise, particularly in resource-poor countries that are already strained by infectious diseases. Presently, cancer therapy relies heavily on chemotherapy and the existing anticancer drugs, which are costly, and have been associated with perilous detrimental systemic side effects in patients. Therefore, development of better and safer alternative methods for cancer treatment and management is urgently required. Regular consumption of fruits and whole grains has been associated with reduced risks of cancer development. Current knowledge on the anticancer activity of Pennisetum glaucum (pearl millet) and Tamarindus indica remains scanty. This study reports the antiproliferative potential of extracts from an indigenous cereal (pearl millet) and citrus fruit (Tamarindus indica) singly and in combination on cervical (Hela), prostate (DU 145) and breast (HCC-1395) cancerous cells.

Methods: The MTT assay was used to ascertain the growth inhibitory potential of the various extracts. Chou-Talalay method was used to ascertain drug interaction of the test samples and the positive control (5-Flouro uracil).

Results: Tamarindus indica whole fruit methanol-dichloromethane had the highest activity against the cell lines tested Hela (IC50-70.24±5.71), Du 145 (IC50-185.54±0.25) and HCC 1395 (IC50-172.58±2.49). Pearl millet methanol-dichloromethane extract gave the best inhibitory activity against Hela (IC50-178.56±1.59), Du 145 – (IC50- 182.16±2.58) and HCC 1395 (IC50346.77±30.00). The combination of pearl millet and tamarind depicted synergistic action against the cancer cells (CI<1). When extracts were combined with 5-flouro uracil (conventional drug) synergism was observed. Additionally, the results obtained indicated that 5-Flouro uracil dosage could be reduced and yet achieve the same effect (DRI>1).

Conclusion: Our findings confirm probable application of Pearl millet and T. indica in cancer control and management. Further studies are recommended to isolate the bioactive compound(s) from the crude extracts and determine the mechanism of action leading to cancer growth inhibition.

Key words: Pearl millet, Tamarind, interaction, synergy, cancer, antiproliferation


Refbacks

  • There are currently no refbacks.