PHYTOCHEMICAL SCREENING AND ANTIPROLIFERATIVE ACTIVITY OF ANNONA MURICATA AND ABELMOSCHUS ESCULENTUS AGAINST BREAST, CERVICAL AND PROSTATE CANCER CELL LINES

Mercy Jepkorir Seroney

Abstract


Cancer is among the leading causes of death in the world. Moreover, cancer treatment has no effective medicine since the current treatment such as chemotherapy and radiotherapy have many side effects and beyond the reach of the common man. Incorporation of fruits and vegetables in diet can successfully be used in prevention and treatment of cancer as they are perceived to be less toxic, readily available and cheap. Annona muricata and Abelmoschus esculentus plants have commonly been used in the traditional medicine to treat various ailments. This study aimed to determine the phytochemical components of both Annona muricata and Abelmoschus esculentus and their antiproliferative activity on breast, cervical and prostate cancer cell line. Method: Methanol and dichloromethane in a ratio of 1:1 was used in extraction. Phytochemical screening was done using standard analytical procedures. The MTT assay was used in antiproliferative assays. The data obtained was tabulated and presented graphically to enhance clarity.

Results: Phytochemical screening showed that the fruit of Annona muricata and the pods of Abelmoschus esculentus are rich in plant secondary metabolites tested viz: saponins, tannins, alkaloids, terpernoids, glycosides, flavonoids and phenols.Annona murica had an IC50 of 25.770µg/ml on Hela (cervical cancer cells), DU145 (Prostate cancer-73.565µg/ml) and HCC 1395 (Breast cancer-94.304µg/ml).Abelmoschus esculentus showed a high antiproliferative activity on Hela with an IC50 of 22.507µg/ml whereas the IC50 of DU145 and HCC 1395 were 50.413 µg/ml and 178.319µg/ml respectively.Both Annona muricata and Abelmoschus esculentus selectively inhibited the growth of the cancerous cells tested (SI>3).

Conclusion: These results suggest that both Annona muricata and Abelmoschus esculentus could be potent agents in cancer management and treatment. The strong antiproliferative activity of   Annona muricata and Abelmoschus esculentus can be attributed to the phytochemicals exhibited by both plant extacts.However,isolation of pure compounds and determination of different growth inhibitory mechanism is recommended


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